Identification and Characterization of Single Chain Anti-cocaine Catalytic Antibodies
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Identification and Characterization of Single-Chain Antibodies that Specifically Bind GI Noroviruses
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We developed anti-Akt1 single-chain antibodies (scFv) by panning a mouse phage–displayed scFv recombinant antibody library. Recombinant scFv that bound glutathione S-transferase (GST)-Akt1 were screened for their ability to inhibit Akt activity in vitro in a kinase reaction containing human recombinant Akt1 and an Akt/serum glucocorticoidinducible kinase (SGK) substrate. Michaelis-Menten analys...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2007
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2006.10.031